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Lower Body Perfusion Reduces the Morbidity of Postoperative Acute Kidney Injury in Type A Dissection: A Propensity-Matched Analysis
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Tang, Zhuo

; Lv, Ying

; Wang, Bin

; Yang, Zhonglu

; Liu, Yu

; Jiang, Hui

Brazilian Journal of Cardiovascular Surgery

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2023, Nº ahead

Resumo: En | Texto: En | PDF: En

ABSTRACT Introduction: Lower body perfusion (LBP) is a technique used to provide blood perfusion to distal organs and spinal cord during circulatory arrest. However, the effect of LBP on the prognosis of aortic arch surgery, especially on postoperative renal function, remains unclear. Methods: A total of 304 patients with acute type A aortic dissection who underwent total aortic arch replacement combined with frozen elephant trunk implantation between May 2016 and December 2021 were retrospectively analyzed. The patients were divided into LBP group (group L, n=85) and non-LBP group (group NL, n=219). Routine lower body circulatory arrest was applied during operation in group NL, and antegrade LBP combined was applied during operation in group L. Perioperative data were recorded. Propensity score matching was used for statistical analysis. Results: After propensity score matching, 85 pairs of patients were successfully matched. Two groups significantly differed in circulatory arrest time (six minutes vs. 30 minutes, P=0.000), cross-clamping time (101 minutes vs. 92 minutes, P=0.010), minimum nasopharyngeal temperature (29.4ºC vs. 27.2ºC, P=0.000), and highest lactate value during cardiopulmonary bypass (2.3 μmol/L vs. 4.1 μmol/L, P=0.000). Considering the postoperative indicators, the drainage volume (450 mL vs. 775 mL, P=0.000) and the incidence of level I acute kidney injury (23.5% vs. 32%, P=0.046) in group L was lower than those in group NL. Conclusion: LBP resulted as a safe and feasible approach in aortic arch surgery, as it could significantly shorten the circulatory arrest time, which might reduce the incidence of postoperative level I acute kidney injury.

https://doi.org/10.21470/1678-9741-2022-0190

INFLUENCES OF ECCENTRIC CONTRACTION TRAINING ON SOCCER PLAYER'S SPEED
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Hou, Kaikai

; Wang, Bin

Revista Brasileira de Medicina do Esporte

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2023, Volume 29 elocation e2022_0608

Resumo: En Es Pt | Texto: En Es Pt | PDF: En | PDF: Es | PDF: Pt

ABSTRACT Introduction: Strength quality can be divided into maximum strength, rapid strength, endurance, and reaction strength. Rapid strength has been a hot topic of discussion in contemporary sports industry research. Objective: Study the influences of eccentric contraction training of the lower limbs in soccer players and its repercussions on the athletes’ speed. Methods: A multifunctional centrifugal training instrument was used as a training tool through the experimental method to analyze the effect of eccentric contraction training on the rapid strength of soccer players. The athletes were divided into experimental and control groups for training. Explosive power, speed sensitivity, and dynamic index of special rapid power were tested before and after training. Results: Compared to the control group, the explosive power index, the sensitivity index, and the kinetic index showed better expressivities in the experimental group; the four jumping ability indexes, the three sensitivity indexes - except the 15s step frequency - and the eccentric training in the experimental group showed a very significant improvement (P<0.01). Conclusion: Eccentric contraction training can effectively improve muscle strength; its training effect is better than that of concentric training, especially eccentric contraction training on the lower limbs, which has an important impact on the rapid strength of soccer players. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

Resumen Introducción: La calidad de la fuerza puede dividirse en fuerza máxima, fuerza rápida, resistencia y fuerza de reacción. Entre ellos, la fuerza rápida ha sido un tema candente de discusión en las investigaciones de la industria deportiva contemporánea. Objetivo: Estudiar las influencias del entrenamiento de la contracción excéntrica de los miembros inferiores en jugadores de fútbol y sus repercusiones en la velocidad de los atletas. Métodos: Se utilizó un instrumento de entrenamiento centrífugo multifuncional como herramienta de entrenamiento a través del método experimental para analizar el efecto del entrenamiento de la contracción excéntrica en la fuerza rápida de los jugadores de fútbol. Los atletas se dividieron en el grupo experimental y el grupo de control para el entrenamiento. La potencia explosiva, la sensibilidad a la velocidad y el índice dinámico de potencia rápida especial se probaron antes y después del entrenamiento. Resultados: En comparación con el grupo de control, el índice de potencia explosiva, el índice de sensibilidad y el índice cinético mostraron mejores expresividades en el grupo experimental; los cuatro índices de capacidad de salto, los tres índices de sensibilidad -excepto la frecuencia de paso de 15s- y el entrenamiento excéntrico en el grupo experimental mostraron una mejora significativa (P<0,01). Conclusión: El entrenamiento de la contracción excéntrica puede mejorar eficazmente la fuerza muscular, su efecto de entrenamiento es mejor que el del entrenamiento concéntrico, especialmente el entrenamiento de la contracción excéntrica en los miembros inferiores, que tiene un impacto importante en la fuerza rápida de los jugadores de fútbol. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

RESUMO Introdução: A qualidade da força pode ser dividida em força máxima, força rápida, resistência e força de reação. Entre elas, a força rápida tem sido um tema de discussão acirrado nas pesquisas da indústria esportiva contemporânea. Objetivo: Estudar as influências do treinamento de contração excêntrica dos membros inferiores em jogadores de futebol e suas repercussões sobre a velocidade dos atletas. Métodos: Utilizou-se um instrumento de treinamento centrífugo multifuncional como ferramenta de treinamento através do método experimental para analisar o efeito do treinamento de contração excêntrica sobre a força rápida dos jogadores de futebol. Os atletas foram divididos no grupo experimental e no grupo de controle para o treinamento. Foram testados a potência explosiva, a sensibilidade à velocidade e o índice dinâmico de potência rápida especial antes e após o treinamento. Resultados: Em comparação com o grupo de controle, o índice de potência explosiva, o índice de sensibilidade e o índice cinético demonstraram melhores expressividades no grupo experimental; os quatro índices de capacidade de salto, os três índices de sensibilidade - exceto a frequência de passos de 15s - e o treinamento excêntrico no grupo experimental mostraram uma melhora significativa (P<0,01). Conclusão: O treinamento de contração excêntrica pode efetivamente melhorar a força muscular, seu efeito de treinamento é melhor do que o do treinamento concêntrico, especialmente o treinamento de contração excêntrica sobre os membros inferiores, que tem um impacto importante sobre a força rápida dos jogadores de futebol. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

https://doi.org/10.1590/1517-8692202329012022_0608

Lower Body Perfusion Reduces the Morbidity of Postoperative Acute Kidney Injury in Type A Dissection: A Propensity-Matched Analysis
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Tang, Zhuo

; Lv, Ying

; Wang, Bin

; Yang, Zhonglu

; Liu, Yu

; Jiang, Hui

Brazilian Journal of Cardiovascular Surgery

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2023, Volume 38 Nº 3 Páginas 346 - 352

Resumo: En | Texto: En | PDF: En

https://doi.org/10.21470/1678-9741-2022-0190

miR-29c-3p represses the angiogenesis of esophageal squamous cell carcinoma by targeting SERPINH1 to regulate the Wnt signaling pathway
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Wei, Desheng

; Ma, Zhifeng

; Zhu, Ting

; Wang, Haiyong

; Wang, Bin

; Fu, Linhai

; Yu, Guangmao

Acta Cirúrgica Brasileira

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2023, Volume 38 elocation e385223

Resumo: En | Texto: En | PDF: En

ABSTRACT Purpose: Esophageal squamous cell carcinoma (ESCC) is characterized by early metastasis and late diagnosis. miR-29c-3p is confirmed to repress angiogenesis in multiple tumor types. Yet, the functions of miR-29c-3p in the mechanism of ESCC angiogenesis, which were not sufficiently explored previously, were exactly what we investigated here at the molecular level. Methods: The mRNA level of miR-29c-3p and Serpin peptidase inhibitor clade H member 1 (SERPINH1) in ESCC tissues were assessed via bioinformatics analysis. Thereafter, miR-29c-3p and SERPINH1 (HSP47) mRNA level in ESCC cell lines was evaluated via quantitative real-time polymerase chain reaction. The effects of abnormal miR-29c-3p and SERPINH1 expression on ESCC cell viability, proliferation, migration, invasion, and HUVEC angiogenesis were examined via CCK8, colony formation, transwell, and angiogenesis assays, respectively. The protein levels of SERPINH1, vascular endothelial growth factor-A (VEGFA), Wnt-1, ?-catenin, and p-?-catenin were evaluated via Western blot. Expression of VEGFA secreted by ESCC cells was measured via enzyme-linked immunosorbent assay. Treatment with the Wnt activator BML-284 further revealed the way miR-29c-3p mediated the Wnt signaling pathway and its effects on angiogenesis. Results: Herein, we revealed a decrease of miR-29c-3p expression in ESCC tissues and cells, while the overexpressed miR-29c-3p could remarkably suppress ESCC cell progression, as well as HUVEC angiogenesis. Meanwhile, overexpressed miR-29c-3p notably downregulated VEGFA and repressed the Wnt signaling pathway. Treatment with the Wnt activator BML-284 could reverse the inhibition of HUVEC angiogenesis caused by miR-29c-3p. SERPINH1 was a downstream target of miR-29c-3p. SERPINH1 knockdown suppressed the malignant phenotypes of ESCC cells and impeded the Wnt signaling activation, while such suppression was reversed through miR-29c-3p inhibitor. Conclusions: We confirmed the mechanism that miR-29c-3p targeted SERPINH1, thus regulating angiogenesis in ESCC through the Wnt signaling pathway. It improves the understanding of angiogenesis in ESCC and offers new ideas for the research of ESCC treatment strategies in the future.

https://doi.org/10.1590/acb385223

MeMYB26, a drought-responsive transcription factor in cassava (Manihot esculenta Crantz)
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Wang, Bin

; Guo, Xin

; Zhao, Pingjuan

; Liao, Wenbin

; Zeng, Changying

; Li, Kai

; Zhou, Yuxun

; Xiao, Junhua

; Ruan, Mengbin

; Peng, Ming

; Bai, Yang

; Chen, Ye

Crop Breeding and Applied Biotechnology

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2021, Volume 21 Nº 1 elocation e34432114

Resumo: En | Texto: En | PDF: En

Abstract Cassava (Manihot esculenta Crantz) is worldwide the sixth-largest food crop and highly drought resistant. The expression of the myeloblastosis (MYB) family protein MeMYB26 in cassava was previously reported to be significantly up-regulated under water deficit. To study the basic characterization, natural variations and potential functions of MeMYB26 for drought resistance in cassava, the protein was subjected to bioinformatics analysis, multiplex-PCR with next-generation sequencing and candidate association studies. The results indicated that MeMYB26 is a typical transcription factor, with two MYB DNA-binding and transcriptional activation domains. One stop-gained and five nonsynonymous variations in the genomic region of MeMYB26 were significantly associated with drought resistance traits. The results of the scale-free coexpression network showed that the MeMYB26 gene plays a critical role in plant stress resistance, growth and biomass development. It was concluded that MeMYB26 is a reliable candidate gene associated with drought tolerance and biomass storage in cassava.

https://doi.org/10.1590/1984-70332021v21n1a4 344 downloads

Baryon Acoustic Oscillations from Integrated Neutral Gas Observations: an instrument to observe the 21cm hydrogen line in the redshift range 0.13 < z < 0.45 – status update
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WUENSCHE, CARLOS A.

; ABDALLA, ELCIO

; ABDALLA, FILIPE

; BAROSI, LUCIANO

; WANG, BIN

; AN, RUI

; BARRETOS, JOÃO A. M.

; BATTYE, RICHARD

; BRITO, FRANCISCO A.

; BROWNE, IAN

; CORREIA, DANIEL

; COSTA, ANDRÉ A.

; DELABROUILLE, JACQUES

; DICKINSON, CLIVE

; FENG, CHANG

; FERREIRA, ELISA G. M.

; FORNAZIER, KARIN

; DE GASPERIS, GIANCARLO

; GUTIERREZ, PRISCILA

; HARPER, STUART

; LANDIM, RICARDO G.

; LICCARDO, VINCENZO

; MA, YIN-ZHE

; MACHADO, TELMO

; MAFFEI, BRUNO

; MARINS, ALESSANDRO

; MENDES, MILENA M. M.

; MERICIA, EDUARDO

; MONSTEIN, CHRISTIAN

; MOTTA, PABLO

; NOVAES, CAMILA

; OTOBONE, CARLOS H.

; PEEL, MICHAEL

; QUEIROZ, AMILCAR R.

; RADCLIFFE, CHRISTOPHER

; REMAZEILLES, MATHIEU

; RIBEIRO, RAFAEL M. G.

; SANG, YU

; SANTOS, JULIANA F. R.

; SANTOS, LARISSA

; SANTOS, MARCELO. V.

; SHAN, CHENXI

; SILVA, GUSTAVO B.

; VIEIRA, FREDERICO

; VIEIRA, JORDANY

; VILLELA, THYRSO

; XIAO, LINFENG

; YANG, WEIQIANG

; ZHANG, JIAJUN

; ZHANG, XUE

; ZHU, ZENGHAO

Anais da Academia Brasileira de Ciências

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2021, Volume 93 elocation e20201096

Resumo: En | Texto: En | PDF: En

Abstract BINGO (BAO from Integrated Neutral Gas Observations) is a unique radio telescope designed to map the intensity of neutral hydrogen distribution at cosmological distances, making the first detection of Baryon Acoustic Oscillations (BAO) in the frequency band 980 MHz - 1260 MHz, corresponding to a redshift range 0.127 < z < 0.449. BAO is one of the most powerful probes of cosmological parameters and BINGO was designed to detect the BAO signal to a level that makes it possible to put new constraints on the equation of state of dark energy. The telescope will be built in Paraíba, Brazil and consists of two \thicksim 40m mirrors, a feedhorn array of 50 horns, and no moving parts, working as a drift-scan instrument. It will cover a 15 ^{\circ} ∘ declination strip centered at \sim \delta ∼ δ =-15 ^{\circ} ∘, mapping \sim ∼ 5400 square degrees in the sky. The BINGO consortium is led by University of São Paulo with co-leadership at National Institute for Space Research and Campina Grande Federal University (Brazil). Telescope subsystems have already been fabricated and tested, and the dish and structure fabrication are expected to start in late 2020, as well as the road and terrain preparation.

https://doi.org/10.1590/0001-3765202120201096 413 downloads

High-precision GPS measurement method without geographical restrictions using crowd-sensing technology
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Zhang, Yunxiang

; Wang, Bin

; Zhang, Lei

Earth Sciences Research Journal

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Dez 2020, Volume 24 Nº 4 Páginas 491 - 497

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

RESUMEN Para mejorar la flexibilidad de la medición GPS se propuso un método de medición GPS de alta precisión que no está restringido por la ubicación geográfica y que se ejecuta bajo tecnología de detección de multitudes. El rendimiento de la red de detección de multitudes se mejoró a través de un mecanismo regular de implementación de redes de detección de macrodatos basado en hexágonos. Se utilizaron los métodos de medición combinados GPS/SINS/DR rápidos para lograr mediciones de alta precisión sin restricciones geográficas. Se ha comprobado que el método propuesto en este trabajo tiene una estabilidad mucho mejor en la estrategia de despliegue del hexágono regular que la del cuadrado. El método propuesto puede lograr una rápida adquisición de señales de satélite y un posicionamiento de alta precisión, y su precisión de medición en ciudades de baja latitud y ciudades de alta latitud es mayor que el método de medición en línea basado en Google Earth, lo que indica que tiene un valor de aplicación significativo.

ABSTRACT In order to improve the flexibility of GPS measurement, a high-precision GPS measurement method that is not restricted by the geographical location under crowd-sensing technology was proposed. The performance of the crowdsensing network was improved through a regular hexagon-based crowd-smart big data sensing network deployment mechanism. The GPS /SINS/DR fast and high-precision combined measurement methods were used to achieve high-precision measurement without geographical restrictions. It has been verified that the proposed method in this paper has much better stability in the deployment strategy of a regular hexagon than that of the square. The proposed method can achieve fast acquisition of satellite signals and high-precision positioning, and its measurement accuracy in the low-latitude city and high-latitude city is higher than the online measurement method based on Google Earth, indicating that it has significant application value.

https://doi.org/10.15446/esrj.v24n4.92151 140 downloads

lncRNA TUG1 promotes proliferation and differentiation of osteoblasts by regulating the miR-545-3p/CNR2 axis
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Hao, Ruizheng

; Wang, Bin

; Wang, Hui

; Huo, Yongxin

; Lu, Yang

Brazilian Journal of Medical and Biological Research

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2020, Volume 53 Nº 11 elocation e9798

Resumo: En | Texto: En | PDF: En

Osteoblast differentiation is an effective way to promote bone formation. Long non-coding RNA taurine upregulated 1 (TUG1) has been identified as a crucial modulator of multiple biological processes. This study was designed to investigate the function of TUG1 in the proliferation and differentiation of osteoblast precursor cells hFOB1.19. In this study, we found that TUG1 promoted hFOB1.19 cell proliferation, while TUG1 knockdown hindered cell proliferation. TUG1 and cannabinoid receptor 2 (CNR2) were upregulated, while miR-545-3p was down-regulated in hFOB1.19 cells undergoing osteoblastic differentiation. TUG1 induced osteoblast differentiation by increasing alkaline phosphatase (ALP) activity and the expression of osteoblastic differentiation markers. TUG1 was a sponge of miR-545-3p and regulated osteoblastic differentiation by modulating miR-545-3p. Moreover, miR-545-3p directly targeted CNR2 and restored the effect of CNR2 on osteoblastic differentiation. In conclusion, TUG1 accelerated the proliferation and differentiation of osteoblasts by sponging miR-545-3p and increasing CNR2 expression, which might provide a new biomarker for bone diseases.

https://doi.org/10.1590/1414-431x20209798 617 downloads

The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis
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Xu, Yuan-kun

; Ke, Yan

; Wang, Bin

; Lin, Jian-hao

Biological Research

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2015, Volume 48 Páginas 1 - 8

Resumo: En | Texto: En | PDF: En

BACKGROUND: Osteoarthritis (OA) is a common arthritic disease and multifactorial whole-joint disease. Interactions of chemokines and OA is inadequately documented RESULTS: In vivo and in vitro studies were conducted to investigate monocyte chemoattractant protein 1 (MCP-1) and receptor chemokine (C-C motif) receptor 2 (CCR2) in chondrocyte degradation and cartilage degeneration. Chondrocytes from 16 OA patients and 6 normal controls were involved in this study. After stimulation of MCP-1, the expression of MCP-1 and CCR2 increased significantly (P < 0.001) and the expression of MMP-13 also increased (P < 0.05). MCP-1 stimulation also induced (or enhanced) the apoptosis of OA chondrocytes (P < 0.05). Additionally, the degradation of cartilage matrix markers (metalloproteinase 3 and 13, MMP3 and MMP13) in the culture medium of normal chondrocytes was also assessed. Furthermore, intra-articular injection of MCP-1 in mouse knees induced cartilage degradation and the CCR2 antagonist did not impede cartilage destroy in rats knees of monosodium iodoacetate (MIA) model CONCLUSIONS: The results of this study demonstrate that the MCP-1-CCR2 ligand-receptor axis plays a special role in the initiation and progression of OA pathology. Patients with ambiguous etiology can gain some insight from the MCP-1-CCR2 ligand-receptor axis

https://doi.org/10.1186/S40659-015-0057-0 1012 downloads

10.

Effects of Dangguibuxue Tang, a Chinese herbal medicine, on growth performance and immune responses in broiler chicks
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Li, Xu Ting

; Wang, Bin

; Li, Jin Liang

; Yang, Rui

; Li, Si Cong

; Zhang, Min

; Huang, Wei

; Cao, Liang

Biological Research

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2013, Volume 46 Nº 2 Páginas 183 - 188

Resumo: En | Texto: En | PDF: En

The effects of Dangguibuxue Tang (DBT) on growth performance and immunity response in immunosuppressed broiler chicks were investigated in this study. 240 one-d-old broiler chicks (DaHeng S01) were randomly divided into 4 groups, 2.0% DBT-treatment (A), 0.5% DBT-treatment (B), cyclophosphamide-control (C), and control group (D). From 4 d to 7 d of age, chicks in group A, B and C were given cyclophosphamide (CY) at a dosage of 100mg/kg body weight (BW) daily by intraperitoneal injection to induce immunosuppression. Chicks in group D were given an equal volume of physiological saline daily by intraperitoneal injection and considered normal chicks. Groups A and B were supplemented with 2.0% or 0.5% of DBT in the drinking water from 8 d to 42 d of age. Groups C and D did not receive any additional medication. The results revealed that chicks from group B had lower feed:gain rate (FGR), lower total mortality, higher immunity organ indexes, higher levels of Newcastle disease (ND) antibody and infectious bursal disease (IBD) antibody, higher interleukin-2 and interleukin-6 levels, and greater lymphocyte proliferative responses to concanavalin A (ConA) during the experiment than those from group C. However, no significant difference in the immunity status in the two levels of DBT-treatment was observed. These results indicate that supplementation of 0.5% of DBT can improve both cellular immunity and humoral immunity in immunosuppressed broiler chicks.

2973 downloads

11.

Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia
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Chen, Liang

; Xia, Hengchuan

; Wang, Yiting

; Chen, Keping

; Qin, Lvgao

; Wang, Bin

; Yao, Qin

; Li, Jun

; He, Yuanqing

; Zhao, Ermi

Genetics and Molecular Biology

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2013, Volume 36 Nº 3 Páginas 438 - 447

Resumo: En | Texto: En | PDF: En

Snake liver has been implicated in the adaptation of snakes to a variety of habitats. However, to date, there has been no systematic analysis of snake liver proteins. In this study, we undertook a proteomic analysis of liver from the colubrid snake Elaphe taeniura using a combination of two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flightmass spectrometry (MALDI-TOF MS). We also constructed a local protein sequence database based on transcriptome sequencing to facilitate protein identification. Of the 268 protein spots revealed by 2-DE 109 gave positive MS signals, 84 of which were identified by searching the NCBInr, Swiss-Prot and local databases. The other 25 protein spots could not be identified, possibly because their transcripts were not be stable enough to be detected by transcriptome sequencing. GO analysis showed that most proteins may be involved in binding, catalysis, cellular processes and metabolic processes. Forty-two of the liver proteins identified were found in other reptiles and in amphibians. The findings of this study provide a good reference map of snake liver proteins that will be useful in molecular investigations of snake physiology and adaptation.

5004 downloads

12.

Perturbations around black holes
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Wang, Bin

Brazilian Journal of Physics

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Dez 2005, Volume 35 Nº 4b Páginas 1029 - 1037

Resumo: En | Texto: En | PDF: En

Perturbations around black holes have been an intriguing topic in the last few decades. They are particularly important today, since they relate to the gravitational wave observations which may provide the unique fingerprint of black holes' existence. Besides the astrophysical interest, theoretically perturbations around black holes can be used as testing grounds to examine the proposed AdS/CFT and dS/CFT correspondence.

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The research on radioprotective agents in Chinese materia medica
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Wang, Bin-Ji

Memórias do Instituto Oswaldo Cruz

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1991, Volume 86 Supl. 2 Páginas 165 - 167

Resumo: En | Texto: En | PDF: En

A series of studies has been carried out in the field of traditional medicine for searching radio-protective agents. According to the theory of traditional Chinese medicine, may prescriptions were tested with experimental animals. Some of them could raise the survival rate of dogs irradiated with lethal dose of Pi-rays by 30-40%. Some symptoms of radiation sickness could be improved. More than one thousand kinds of Chinese herbs were screened. Some of them have pronounced radioprotectice activities. A series of bioactive components wee isolated from these herbs. The mechanism of radiation protection were studied. Having the capability of hemopoietic system and immune system may be the characteristics of these Chinese herbs.

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